Aleftina Konstantinova joined the troupe of the Oryol Regional Drama Theatre when she was just 16 years old. She graduated from the acting department of the State Institute of Theatre Arts in 1959, after which she was immediately invited to join the company at the Stanislavsky Drama Theatre. In her first two seasons she played the lead female roles in: Love Like That, Meetings on the Road, and Anya Beryozko's Love. Her first serious success, however, came with her performance of Nina Zarechnaya in The Seagull. She received the grand prize for best female performance at the Pan Union Chekhov Festival.
While serving in the same theatre for 50 years, Aleftina Konstantinova has played the roles of Ismene (Antigone, directed by Boris Lvov-Anokhin), Anna Vasilyeva (A Housewarming in an Old House, directed by Alexander Tovstonogov), IOlan (The Devil, directed by Les Tanyuk) and others.
Konstantinova has played many memorable roles in cinema. She debuted on screen as a student in 1957, playing Tanya in A Sailor Came on Shore. Other roles include Vera in After the Wedding, Masha in the dramatic film One-Street Town, Valentina Kolomiitseva in The Light of a Distant Star, directed by the great Ivan Pyryev.
Aleftina Konstantinova works in the Stanislavsky Electrotheatre alongside her husband Vladimir Korenev and her daughter Irina Koreneva.